APPLICATION NOTE - RAPID EXOME SEQUENCING USING THE ION PROTON® SYSTEM AND ION AMPLISEQ™ TECHNOLOGY
Now Ion AmpliSeq™ technology has been expanded to target the human exome to create an easy-to-implement, cost-effective, flexible exome sequencing workflow using the Ion Proton® System with the Ion PI™ Chip, and data analysis using Ion Reporter™ Software (Figure 1A). The simplicity and speed of PCR allows the most rapid method of exome enrichment with a total library construction time of <6 hours, requiring the least amount of hands-on time and the fewest pipetting steps compared to alternative methods (Figure 1B, Nextera® Rapid Exome Capture Kit*, HaloPlex® Exome Kit**).
*Values obtained from Illumina Nextera® Rapid Capture Exome Data Sheet
**Values obtained from Agilent Technologies HaloPlex® Exome Data Sheet and hands-on time estimated from the protocol